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24 Investigating the Lymphatic Drainage of the Brain: .. Several manufacturers of manual pipettes (Including Gilson, Mettler Toledo and Eppendorf) P2. P3. P4. P5. Fig. 5 Monocyte, monocyte-derived DC, macrophages gating strategy Cells were prepared from skin and analyzed by flow cytometry (see Table 2).
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Single cells were manually aspirated using a Pipetman P2 (Gilson, Inc, Middleton, Wis.) under visual guidance. Cells were collected in 1 ?l volume and added to 0.2 ?l of SUPERase-In™ RNAse inhibitor (Applied Biosystems/Ambion, Austin, Tex.) and frozen on dry ice. Samples were stored at minus 80° C. until processed.
Product Designation, Ordering reference, PIPETMAN Models, DIANOND Tips. Starter Kit, F167300, P20 - P200 - P1000, D200 - D1000. Micro-volume Kit, F167500, P2 - P10 - P100, DFL10ST - DF100ST. Large-volume Kit, F167400, P5000 - P10 mL, D5000 - D10 mL
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PIPETMAN® P. 4 - OPERATING RANGES. Model. Range*. P2. 0.2 - 2 µL. P10. 1 - 10 µL. P20. 2 - 20 µL. P100. 20 - 100 µL. P200. 50 - 200 µL. P1000. 200 - 1000 µL. P5000. 1 - 5 mL. P10ml. 1 - 10 mL. * With a precise pipetting technique (see “General. Guidelines for good pipetting”) P2 may be used to aspirate volumes
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circumstances, and this manual makes reference to integrated measles-rubella surveillance. Member . Primary viraemia. 3-5. Multiplication of measles virus in respiratory epithelium, regional lymph nodes and distant sites pipette to avoid extracting red cells, and transferred aseptically to a sterile labelled vial with the
PIPETMAN P is a fully adjustable, air-displacement pipette with the selected volume shown on a digital indicator (volumeter). Eight models cover a range from 0.2 µL to 10 mL, for many applications: P2 and P10: measurement and transfer of micro- volumes, DNA sequencing and enzyme-assays. P20, P100, P200, P1000:
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